fluorometric kit Search Results


apoptosis kit  (Novus Biologicals)
86
Novus Biologicals apoptosis kit
Apoptosis Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pmc06345847-245-61-64?v=Novus+Biologicals
Average 86 stars, based on 1 article reviews
apoptosis kit - by Bioz Stars, 2026-07
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extracellular acidification rate ecar fluorometric assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology extracellular acidification rate ecar fluorometric assay kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Extracellular Acidification Rate Ecar Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pmc12858680-70-0-11?v=Elabscience+Biotechnology
Average 95 stars, based on 1 article reviews
extracellular acidification rate ecar fluorometric assay kit - by Bioz Stars, 2026-07
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cystine uptake fluorometric assay kit  (Elabscience Biotechnology)
94
Elabscience Biotechnology cystine uptake fluorometric assay kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Cystine Uptake Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pmc12870982-46-7-16?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
cystine uptake fluorometric assay kit - by Bioz Stars, 2026-07
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cell ferrous iron fe2 fluorometric assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology cell ferrous iron fe2 fluorometric assay kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Cell Ferrous Iron Fe2 Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pm40999865-503-17-25?v=Elabscience+Biotechnology
Average 95 stars, based on 1 article reviews
cell ferrous iron fe2 fluorometric assay kit - by Bioz Stars, 2026-07
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ocr fluorometric assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology ocr fluorometric assay kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Ocr Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pmc12381239-253-28-34?v=Elabscience+Biotechnology
Average 95 stars, based on 1 article reviews
ocr fluorometric assay kit - by Bioz Stars, 2026-07
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reactive oxygen species ros estimation ros fluorometric test kit  (Elabscience Biotechnology)
96
Elabscience Biotechnology reactive oxygen species ros estimation ros fluorometric test kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Reactive Oxygen Species Ros Estimation Ros Fluorometric Test Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/10__1186_slash_s41936___025___00450___9-101-0-9?v=Elabscience+Biotechnology
Average 96 stars, based on 1 article reviews
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rat alt elisa assay kit  (Elabscience Biotechnology)
93
Elabscience Biotechnology rat alt elisa assay kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Rat Alt Elisa Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pmc09864559-84-8-18?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
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malondialdehyde mda  (Elabscience Biotechnology)
93
Elabscience Biotechnology malondialdehyde mda
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Malondialdehyde Mda, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pm40579608-89-12-14?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
malondialdehyde mda - by Bioz Stars, 2026-07
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cholesteryl ester fluorometric assay kit  (Elabscience Biotechnology)
93
Elabscience Biotechnology cholesteryl ester fluorometric assay kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Cholesteryl Ester Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/pmc09658088-42-8-13?v=Elabscience+Biotechnology
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cholesteryl ester fluorometric assay kit - by Bioz Stars, 2026-07
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glucose uptake test kit  (Elabscience Biotechnology)
94
Elabscience Biotechnology glucose uptake test kit
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Glucose Uptake Test Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/10__1289_slash_ehp14888-240-3-9?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
glucose uptake test kit - by Bioz Stars, 2026-07
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hydrogen peroxide assay  (Elabscience Biotechnology)
94
Elabscience Biotechnology hydrogen peroxide assay
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
Hydrogen Peroxide Assay, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/10__3390_slash_antiox14060671-85-5-8?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
hydrogen peroxide assay - by Bioz Stars, 2026-07
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e bc f003  (Elabscience Biotechnology)
93
Elabscience Biotechnology e bc f003
PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) <t>Extracellular</t> <t>acidification</t> <t>rate</t> in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6
E Bc F003, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) Extracellular acidification rate in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: PRC1 promotes immunosuppressive macrophages in sepsis via β-catenin/STAT3 signaling

doi: 10.1007/s00018-025-06070-4

Figure Lengend Snippet: PRC1 knockdown restores inflammatory response in endotoxin-tolerant macrophages. ( A , B ) Relative mRNA expression of TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in naive and endotoxin-tolerant THP-1, with or without LPS stimulation (100ng/mL) for 6 h. n = 6. ( C , D ) Relative mRNA and protein expression of PRC1 in THP-1 after transfected with siRNA-358, siRNA-631 and siRNA-1001. n = 3–6. ( E ) Relative mRNA expression of PRC1 in endotoxin-tolerant THP-1 after transfected with PRC1-siRNA. n = 6. ( F , G ) Relative mRNA expression of IL-1β, TNF-α, IL-6, TGF-β, IL-10, CD86 and CD206 in resting or LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( H ) Relative mRNA expression of HLA-DRA and CD74 in resting or LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( I ) HLA-DR expression levels identified by flow cytometry in LPS-rechallenged endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 3. ( J ) Extracellular acidification rate in LPS-stimulated naïve or endotoxin-tolerant THP-1. n = 8. ( K ) Relative protein expression of HIF-1α in LPS-stimulated endotoxin-tolerant THP-1, with or without PRC1 knockdown. n = 6

Article Snippet: Extracellular acidification rate (ECAR) Fluorometric Assay Kit (Cat#E-BC-F069) was purchased from Elabscience (Wuhan, China).

Techniques: Knockdown, Expressing, Transfection, Flow Cytometry